p53-gfp fusion protein-expressing construct Search Results


p53-gfp fusion protein-expressing construct  (SpectraGenetics Inc)
90
SpectraGenetics Inc p53-gfp fusion protein-expressing construct
Live-cell imaging of <t>p53-GFP-expressing</t> Saos-2 cells. Saos-2 cells were transfected as described previously with pJCPAC-Mam1-p53-GFP. Three days post-transfection, Saos-2 cells were photographed and UV and white light images were merged. (A). Exogenous p53-GFP localizes predominantly to the nuclei of Saos-2 cells. (B). Membrane blebbing, a hallmark event in apoptosis occurs in a subset of p53-GFP-expressing Saos-2 cells.
P53 Gfp Fusion Protein Expressing Construct, supplied by SpectraGenetics Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Live-cell imaging of p53-GFP-expressing Saos-2 cells. Saos-2 cells were transfected as described previously with pJCPAC-Mam1-p53-GFP. Three days post-transfection, Saos-2 cells were photographed and UV and white light images were merged. (A). Exogenous p53-GFP localizes predominantly to the nuclei of Saos-2 cells. (B). Membrane blebbing, a hallmark event in apoptosis occurs in a subset of p53-GFP-expressing Saos-2 cells.

Journal: Gene

Article Title: An arrayed human genomic library constructed in the PAC shuttle vector pJCPAC-Mam2 for genome-wide association studies and gene therapy

doi: 10.1016/j.gene.2012.01.011

Figure Lengend Snippet: Live-cell imaging of p53-GFP-expressing Saos-2 cells. Saos-2 cells were transfected as described previously with pJCPAC-Mam1-p53-GFP. Three days post-transfection, Saos-2 cells were photographed and UV and white light images were merged. (A). Exogenous p53-GFP localizes predominantly to the nuclei of Saos-2 cells. (B). Membrane blebbing, a hallmark event in apoptosis occurs in a subset of p53-GFP-expressing Saos-2 cells.

Article Snippet: A p53-GFP cassette (Gift of Jon Jarvik, SpectraGenetics) was subcloned into the unique NotI site of pJCPAC-Mam1 and Saos-2 cells were also transfected with this p53-GFP fusion protein-expressing construct using PEI as previously described.

Techniques: Live Cell Imaging, Expressing, Transfection